| I am
now leading an ocean realm field project of the Census
of Marine Life (CoML), the Census of Marine Zooplankton
(CMarZ, see www.CMarZ.org), which will complete a global
survey of zooplankton biodiversity by 2010. As part
of this effort, we are working on molecular phylogenetic
and phylogeographic analyses of diverse groups, focusing
on crustacean and gelatinous taxa. CMarZ has also allowed
us to explore speciation patterns and processes in zooplankton.
Recent studies have compared molecular and morphological
phylogenies for copepods and euphausiids, revealed
cryptic species within circumglobal taxa, and used
PCR-based protocols to accurately assess species diversity
of targeted ocean regions.
Some
Representative Publications
Papetti, C., L. Zane, E. Bortolotto, A. Bucklin, and T.
Patarnello. 2005. Genetic differentiation and local temporal
stability of population structure in the euphausiid Meganyctiphanes
norvegica. Mar. Ecol. Prog. Ser. 289:
225 – 235.
Manning, C.A. and A. Bucklin. 2005. Multivariate analysis
of the copepod community of near-shore waters in the western
Gulf of Maine (Northwest Atlantic). Mar. Ecol.
Prog. Ser. 292: 233-249
Bucklin, A. and L.D. Allen. 2004. MtDNA sequencing from
zooplankton after long-term preservation in buffered formalin.
Molec. Phylog. Evol. 30: 879-882. (PDF)
Caudill, C.C. and A. Bucklin. 2004. Molecular phylogeography
and evolutionary history of the estuarine copepod, Acartia
tonsa, on the Northwest Atlantic coast. Hydrobiol.
511: 91-102. (PDF)
Bucklin, A., B.W. Frost, J. Bradford Grieve, L.D. Allen,
and N.J. Copley. 2003. Molecular systematic assessment
of thirty-four calanoid copepod species of the Calanidae
and Clausocalanidae using DNA sequences of mtCOI and nuclear
18S rRNA. Mar. Biol. 142: 333-343. (PDF)
Wiebe, P.H., R. Beardsley, D. Mountain, and A. Bucklin.
2002. U.S. GLOBEC Atlantic / Georges Bank Program. Oceanography
15(2): 13-29.
McGillicuddy, D. J. Jr. and A. Bucklin. 2002. Intermingling
of two Pseudocalanus species on Georges Bank. J.
Mar. Res. 60: 583-604. (PDF)
Bucklin, A., P.H. Wiebe, S.B. Smolenack, and M.E. Clarke.
2002. Integrated biochemical, molecular genetic, and bioacoustical
analysis of mesoscale variability of the euphausiid Nematoscelis
difficilis in the California Current. Deep-Sea
Res. 49: 437-462. (PDF)
Bucklin, A. et al. 2001. FleetLink: Collection and telemetry
of ocean and weather data. Sea Technology,
May, 2001
Hill, R.S., L.D. Allen, and A. Bucklin. 2001. Multiplexed
species-specific PCR protocol to discriminate four N.
Atlantic Calanus species, with a mtCOI gene tree for ten
Calanus species. Mar. Biol. 139: 279-287.
(PDF)
Wiebe, P.H., R.C. Beardsley, A. Bucklin, and D.G. Mountain.
2001. Coupled biological and physical studies of plankton
populations in the Georges Bank region and related North
Atlantic GLOBEC sites. Deep-Sea Res. 48:
1-2.
Bucklin, A., M. Guarnieri, D.J. McGillicuddy, and R.S.
Hill. 2001. Spring evolution of Pseudocalanus spp. abundance
on Georges Bank based on molecular discrimination of P.
moultoni and P. newmani . Deep-Sea Res.
48: 589-609. (PDF)
Bucklin, A., P.H. Wiebe, O.S. Astthorsson, A. Gislason,
L.D. Allen, and S.B. Smolenack. 2000. Population genetic
variation of C. finmarchicus in Icelandic waters: preliminary
evidence of genetic differences between Atlantic and Polar
populations. ICES J. Mar. Res. 57:1592-1604.
(PDF)
Bucklin, A., S. Kaartvedt, M. Guarnieri, and U. Goswami.
2000. Population genetics of drifting ( Calanus spp.)
and resident ( Acartia clausi ) plankton in Norwegian
fjords. J. Plank. Res. 22: 1237-1251.
Bucklin, A. 2000. Methods for Population Genetic Analysis
of Zooplankton. Chapter 11 in: The Zooplankton Methodology
Manual , International Council for the Exploration of
the Sea. Academic press, London. Pp.
533 - 570. (PDF)
Bucklin, A. and W.H. Howell. 1999. Progress and prospects
from the UNH Open Ocean Aquaculture Demonstration Project.
In: R.R. Stickney (Ed.), Joining Forces with Industry,
Proceedings of the Third International Conference
on Open Ocean Aquaculture , Corpus Christi, TX,
May 10 - 15, 1998. Pp. 7 - 30.
Bucklin, A., R.S. Hill, and M. Guarnieri. 1999. Taxonomic
and systematic assessment of planktonic copepods using
mitochondrial COI sequence variation and competitive,
species-specific PCR. Special Issue on Molecular Ecology
of Aquatic Communities (J.P. Zehr and M. Voytek, Eds.)
Hydrobiol. 401:239-254.
Bucklin, A. and P.H. Wiebe. 1998. Low mitochondrial diversity
and small effective population sizes of the copepods,
Calanus finmarchicus and Nannocalanus minor : possible
impact of climatic variation during recent glaciation.
J. Heredity 89: 383-392. (PDF)
Bucklin, A. 1998. Molecular analysis of biogeographic
patterns and processes: the copepod Nannocalanus minor
in the N. Atlantic Ocean. In: Proceedings of the
Second International Conference on Pelagic Biogeography
, 9-14 July 1995. Intergovernmental Oceanographic Comm.
Workshop Rpt. No. 142: 78-85.
Bucklin, A., C.C. Caudill, and M. Guarnieri. 1998. Population
genetics and phylogeny of marine planktonic copepods.
In: Molecular Approaches to the Study of the Ocean
, Chapter 14. K.C. Cooksey (ed.). Chapman & Hall,
London, UK. Pp. 303-317.
Bucklin, A., A.M. Bentley, and S.P. Franzen. 1998. Distribution
and relative abundance of the copepods, Pseudocalanus
moultoni and P. newmani , on Georges Bank based on molecular
identification of sibling species. Mar. Biol.
132: 97-106.
Bucklin, A. , S.B. Smolenack, A.M. Bentley, and P.H. Wiebe.
1997. Gene flow patterns of the euphausiid, Meganyctiphanes
norvegica , in the N. Atlantic based on DNA sequences
for mitochondrial cytochrome oxidase I and cytochrome
b. J. Plank. Res. 19:1763-1781.
Bucklin, A., R.S. Hill, M. Guarnieri. 1997. Seasonal patterns
of distribution and abundance of the copepods, Pseudocalanus
moultoni and P. newmani , on Georges Bank: evidence for
a dynamic balance between retention and loss. Background
paper, ICES CM 1997/T:06.
Madin, L.P. , S.M. Bollens, E. Horgan, M. Butler, J. Runge,
B.K. Sullivan, G. Klein-MacPhee, E. Durbin, A.G. Durbin,
A. Bucklin, and M. E. Clarke. 1996. Voracious planktonic
hydroids: unexpected predatory impact on a coastal marine
ecosystem. Deep-Sea Res. 43: 1823-1830.
Bucklin, A. and T.D. Kocher. 1996. Source regions for
recruitment of Calanus finmarchicus to Georges Bank: evidence
from molecular population genetic analysis of mtDNA. Deep-Sea
Res. 43: 1665-1682.
Wiebe, P.H., D. Mountain, R. Beardsley, and A. Bucklin.
1996. Global Ocean Ecosystem Dynamics - initial program
in Northwest Atlantic. Sea Technology
37(8): 67-76.
Bucklin, A., R. Sundt, and G. Dahle. 1996. Population
genetics of Calanus finmarchicus in the North Atlantic.
Proceedings of a I.C.E.S. planning workshop for a TransAtlantic
Study of Calanus finmarchicus . Ophelia 44: 29-45.
Bucklin, A., T.C. LaJeunesse, E. Curry, J. Wallinga, and
K. Garrison. 1996. Molecular genetic diversity of the
copepod, Nannocalanus minor: genetic evidence of species
and population structure in the N. Atlantic Ocean. J.
Marine Res. 54: 285-310.
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including
DNA sequence variation of mitochondrial genes, Single
Nucleotide Polymorphisms (SNPs) in coding and non-coding
(intron) regions of nuclear genes, allele-specific PCR,
and analysis of microsatellite DNA - to examine geographic
variation of zooplankton species with extensive and/or
disjunct distributions.