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My laboratory uses diverse molecular approaches and protocols to study the ecology, systematics, and evolution of marine holozooplankton, especially copepods and euphausiids.
We study the molecular population genetic diversity and structure of zooplankton using DNA sequence variation of mitochondrial genes, Single Nucleotide Polymorphisms (SNPs) in coding and non-coding (intron) regions of nuclear genes, and microsatellite DNA. Our goals are to examine geographic variation of zooplankton populations over spatial scales ranging from small-scale patches to ocean-basin gyres.
We study patterns of gene expression in zooplankton using quantitative real-time PCR (QPCR) of target genes encoding metabolic enzymes and environmental stress response proteins, as well as EST (expressed sequence tag) microarrays to characterize genome-wide responses of zooplankton to biological and physical environmental conditions. As an initial step in working with new species, we build cDNA and genomic libraries to develop molecular databases to mine for molecular markers and target genes.
We are working to analyze the evolutionary history and
molecular phylogeny of copepods and euphausiids using multi-gene sequences. Our molecular phylogeny will be compared with results of morphological phylogenetic analyses (carried out by collaborating expert taxonomists). We hope to create improved understanding of the timing and mode of speciation of these ecologically important and taxonomically complex zooplankton groups.
The Census of Marine Zooplankton (CMarZ, see www.CMarZ.org) is an ocean realm field project of the Census of Marine Life (CoML), which is a primary focus of our research efforts. The goal of CMarZ is to complete a global survey of zooplankton biodiversity. In particular, we are characterizing global patterns of species diversity using DNA
barcodes (i.e., short DNA sequences for species recognition and
discrimination).
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Research Projects
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Environmental Genomics of Salpa thompsoni in the Southern Ocean - National Science Foundation / Office of Polar Programs
Climate forcing of Calanus finmarchicus populations of the North Atlantic. - National Science Foundation / U.S. GLOBEC Synthesis
Census of Marine Zooplankton (CMarZ); a global survey of holozooplankton biodiversity with molecular systematic analysis - Alfred P. Sloan Foundation
Trophic Structure and Interactions of the Pelagic Nekton Assemblage - National Science Foundation / Biological Oceanography
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Current Students
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| Paola Gabriela Batta Lona - Ph.D. Student |
| Ebru Unal Yigiterhan - Ph.D. Student |
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Publications
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Bucklin, A., R.R. Hopcroft, K.N. Kosobokova, L.M. Nigro, B.D. Ortman, R.M. Jennings, and C.J. Sweetman. 2009. DNA barcoding of Arctic Ocean holozooplankton for species identification and recognition. Deep-Sea Res. (in press)
Bucklin, A. and B.W. Frost. 2009. Morphological and molecular phylogenetic analysis of evolutionary lineages within Clausocalanus (Crustacea, Copepoda, Calanoida). J. Crustacean Biol. 29: 111-120.
Bucklin, A., P.H. Wiebe, S.B. Smolenack, N.J. Copley, J.G. Beaudet, K.G. Bonner, J. Färber Lorda, and J.J. Pierson. 2007. DNA barcodes for species identification of euphausiids (Euphausiacea, Crustacea). J. Plankton Res. 29: 483-493.
Ueda H. and A. Bucklin. 2006. Acartia (Odontacartia) ohtsukai, a newbrackish-water calanoid copepod from Ariake Bay, Japan, with a redescription of the closely related A. pacifica from the Seto Inland Sea. Hydrobiol. 500: 77-91.
Papetti, C., L. Zane, E. Bortolotto, A. Bucklin, and T. Patarnello. 2005. Genetic differentiation and local temporal stability of population structure in the euphausiid Meganyctiphanes norvegica. Mar. Ecol. Progr. Ser. 289: 225 – 235.
Manning, C.A. and A. Bucklin. 2005. Multivariate analysis of the copepod community of near-shore waters in the western Gulf of Maine (Northwest Atlantic). Mar. Ecol. Progr. Ser. 292: 233-249
Bucklin, A. and L.D. Allen. 2004. MtDNA sequencing from zooplankton after long-term preservation in buffered formalin. Molec. Phylog. Evol. 30: 879-882.
Caudill, C.C. and A. Bucklin. 2004. Molecular phylogeography and evolutionary history of the estuarine copepod, Acartia tonsa, on the Northwest Atlantic coast. Hydrobiol. 511: 91-102.
Bucklin, A., B.W. Frost, J. Bradford Grieve, L.D. Allen, and N.J. Copley. 2003. Molecular systematic assessment of thirty-four calanoid copepod species of the Calanidae and Clausocalanidae using DNA sequences of mtCOI and nuclear 18S rRNA. Mar. Biol. 142: 333-343.
Wiebe, P.H., R. Beardsley, D. Mountain, and A. Bucklin. 2002. U.S. GLOBEC Atlantic / Georges Bank Program. Oceanography 15(2): 13-29.
McGillicuddy, D. J. Jr. and A. Bucklin. 2002. Intermingling of two Pseudocalanus species on Georges Bank.J. Mar. Res. 60: 583-604.
Bucklin, A., P.H. Wiebe, S.B. Smolenack, and M.E. Clarke. 2002. Integrated biochemical, molecular genetic, and bioacoustical analysis of mesoscale variability of the euphausiid Nematoscelis difficilis in the California Current. Deep-Sea Res. 49: 437-462.
Bucklin, A. et al. 2001. FleetLink: Collection and telemetry of ocean and weather data. Sea Technology, May, 2001
Hill, R.S., L.D. Allen, and A. Bucklin. 2001. Multiplexed species-specific PCR protocol to discriminate four N. Atlantic Calanus species, with a mtCOI gene tree for ten Calanus species. Mar. Biol. 139: 279-287.
Wiebe, P.H., R.C. Beardsley, A. Bucklin, and D.G. Mountain. 2001. Coupled biological and physical studies of plankton populations in the Georges Bank region and related North Atlantic GLOBEC sites. Deep-Sea Res. 48: 1-2.
Bucklin, A., M. Guarnieri, D.J. McGillicuddy, and R.S. Hill. 2001. Spring evolution of Pseudocalanus spp. abundance on Georges Bank based on molecular discrimination of P. moultoni and P. newmani . Deep-Sea Res. 48: 589-609.
Bucklin, A., P.H. Wiebe, O.S. Astthorsson, A. Gislason, L.D. Allen, and S.B. Smolenack. 2000. Population genetic variation of C. finmarchicus in Icelandic waters: preliminary evidence of genetic differences between Atlantic and Polar populations. ICES J. Mar. Res. 57:1592-1604.
Bucklin, A., S. Kaartvedt, M. Guarnieri, and U. Goswami. 2000. Population genetics of drifting ( Calanus spp.) and resident ( Acartia clausi ) plankton in Norwegian fjords. J. Plank. Res. 22: 1237-1251.
Bucklin, A. 2000. Methods for Population Genetic Analysis of Zooplankton. Chapter 11 in: The Zooplankton Methodology Manual , International Council for the Exploration of the Sea. Academic Press, London. Pp. 533 - 570.
Bucklin, A. and W.H. Howell. 1999. Progress and prospects from the UNH Open Ocean Aquaculture Demonstration Project. In: R.R. Stickney (Ed.), Joining Forces with Industry, Proc. Third Internat. Conf. Open Ocean Aquaculture , Corpus Christi, TX, May 10 - 15, 1998. Pp. 7 - 30.
Bucklin, A., R.S. Hill, and M. Guarnieri. 1999. Taxonomic and systematic assessment of planktonic copepods using mitochondrial COI sequence variation and competitive, species-specific PCR. Special Issue on Molecular Ecology of Aquatic Communities (J.P. Zehr and M. Voytek, Eds.) Hydrobiol. 401:239-254.
Bucklin, A. and P.H. Wiebe. 1998. Low mitochondrial diversity and small effective population sizes of the copepods, Calanus finmarchicus and Nannocalanus minor : possible impact of climatic variation during recent glaciation. J. Heredity 89: 383-392.
Bucklin, A. 1998. Molecular analysis of biogeographic patterns and processes: the copepod Nannocalanus minor in the N. Atlantic Ocean. In: Proceedings of the Second International Conference on Pelagic Biogeography , 9-14 July 1995. Intergovernmental Oceanographic Comm. Workshop Rpt. No. 142: 78-85.
Bucklin, A., C.C. Caudill, and M. Guarnieri. 1998. Population genetics and phylogeny of marine planktonic copepods. In: Molecular Approaches to the Study of the Ocean , Chapter 14. K.C. Cooksey (ed.). Chapman & Hall, London, UK. Pp. 303-317.
Bucklin, A., A.M. Bentley, and S.P. Franzen. 1998. Distribution and relative abundance of the copepods, Pseudocalanus moultoni and P. newmani , on Georges Bank based on molecular identification of sibling species. Mar. Biol. 132: 97-106.
Bucklin, A. , S.B. Smolenack, A.M. Bentley, and P.H. Wiebe. 1997. Gene flow patterns of the euphausiid, Meganyctiphanes norvegica , in the N. Atlantic based on DNA sequences for mitochondrial cytochrome oxidase I and cytochrome b. J. Plank. Res. 19:1763-1781.
Bucklin, A., R.S. Hill, M. Guarnieri. 1997. Seasonal patterns of distribution and abundance of the copepods, Pseudocalanus moultoni and P. newmani , on Georges Bank: evidence for a dynamic balance between retention and loss. ICES Ann. Science Conf. Proc. 1997/T:06.
Madin, L.P. , S.M. Bollens, E. Horgan, M. Butler, J. Runge, B.K. Sullivan, G. Klein-MacPhee, E. Durbin, A.G. Durbin, A. Bucklin, and M. E. Clarke. 1996. Voracious planktonic hydroids: unexpected predatory impact on a coastal marine ecosystem. Deep-Sea Res. 43: 1823-1830.
Bucklin, A. and T.D. Kocher. 1996. Source regions for recruitment of Calanus finmarchicus to Georges Bank: evidence from molecular population genetic analysis of mtDNA. Deep-Sea Res. 43: 1665-1682.
Wiebe, P.H., D. Mountain, R. Beardsley, and A. Bucklin. 1996. Global Ocean Ecosystem Dynamics - initial program in Northwest Atlantic. Sea Technology 37(8): 67-76.
Bucklin, A., R. Sundt, and G. Dahle. 1996. Population genetics of Calanus finmarchicus in the North Atlantic. Ophelia 44: 29-45.
Bucklin, A., T.C. LaJeunesse, E. Curry, J. Wallinga, and K. Garrison. 1996. Molecular genetic diversity of the copepod, Nannocalanus minor: genetic evidence of species and population structure in the N. Atlantic Ocean. J. Marine Res. 54: 285-310.
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Ann Bucklin |
| University of Connecticut Department of Marine Sciences |
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| 1080 Shennecossett Road Groton, CT 06340 |
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| Phone: (860) 405-9208 |
| Fax: (860) 405-9153 |
| email: ann.bucklin@uconn.edu |
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